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Merck & Co assay data analyzer software
Assay Data Analyzer Software, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/analyzer+assay+data+software/pm24900466__ml200272z_si_001-52-4-8
Average 86 stars, based on 1 article reviews
assay data analyzer software - by Bioz Stars, 2026-10
86/100 stars

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Article Title: Supporting Information Inhibition of Glucose Dependent Insulin Secretion by a Potent, Selective sst3 Antagonist
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.). ..

Article Title: Supporting Information Diamine Derivatives as Novel Small-Molecule, Potent, and Subtype- Selective Somatostatin SST3 Receptor Agonists
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.) SST cAMP Functional Assay Method: The cAMP agonist potency determinations were made using a modification of the PerkinElmer Lance 384 kit (Cat#AD0264). ..

Article Title: Supporting Information Diamine Derivatives as Novel Small-Molecule, Potent, and Subtype- Selective Somatostatin SST3 Receptor Agonists
Article Snippet: Plates were incubated for 45 minutes at 37°C and 5% CO2 followed by addition of 12 μL detection mix for 1 h incubation at room temperature Time-resolved fluorescence was measured using Envision plate reader (PerkinElmer Life and Analytical Sciences, Inc. Waltham, MA) at 665 nm and 615 nm. .. Resulting cAMP levels were calculated by reading the relative fluorescence units off a cAMP standard curve then agonist EC50s were subsequently determined from 4- parameter fits by plotting those cAMP levels versus agonist concentration using Assay Data Analyzer software (Merck and Co.). ..

Article Title: SUPPORTING INFORMATION Discovery of MK-1421, a Potent, Selective sstr3 Antagonist, as a Development Candidate for Type 2 Diabetes
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.) .. The cAMP agonist potency determinations were made using a modification of the PerkinElmer Lance 384 kit (Cat#AD0264).

Article Title: Investigation of Cardiovascular Effects of Tetrahydro-β-carboline sstr3 antagonists Supporting Information
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.) sstr cAMP Functional Assay Method: The cAMP agonist potency determinations were made using a modification of the PerkinElmer Lance 384 kit (Cat#AD0264). ..

Article Title: The Discovery of MK-4256, a Potent SSTR3 Antagonist as a Potential Treatment of Type-2 Diabetes Supporting Information
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.) SSTR cAMP Functional Assay Method: The cAMP agonist potency determinations were made using a modification of the PerkinElmer Lance 384 kit (Cat#AD0264). ..

Article Title: The Discovery of MK-4256, a Potent SSTR3 Antagonist as a Potential Treatment of Type-2 Diabetes Supporting Information
Article Snippet: Plates were incubated for 45 minutes at 37°C and 5% CO2 followed by addition of 12 μL detection mix for 1 h incubation at room temperature Time-resolved fluorescence was measured using Envision plate reader (PerkinElmer Life and Analytical Sciences, Inc. Waltham, MA) at 665 nm and 615 nm. .. Resulting cAMP levels were calculated by reading the relative fluorescence units off a cAMP standard curve then agonist EC50s were subsequently determined from 4-parameter fits by plotting those cAMP levels versus agonist concentration using Assay Data Analyzer software (Merck and Co.). .. Protocols for ipGTT and oGTT Mouse oGTT procedure C57BL/6 mice (Taconic), 12 weeks old were fasted 6 hrs.

Article Title: Investigation of Cardiovascular Effects of Tetrahydro-β-carboline sstr3 antagonists Supporting Information
Article Snippet: Plates were incubated for 45 minutes at 37°C and 5% CO2 followed by addition of 12 μL detection mix for 1 h incubation at room temperature Time-resolved fluorescence was measured using Envision plate reader (PerkinElmer Life and Analytical Sciences, Inc. Waltham, MA) at 665 nm and 615 nm. .. Resulting cAMP levels were calculated by reading the relative fluorescence units off a cAMP standard curve then agonist EC50s were subsequently determined from 4-parameter fits by plotting those cAMP levels versus agonist concentration using Assay Data Analyzer software (Merck and Co.). .. Protocol for mouse oGTT C57BL/6 mice (Taconic), 12 weeks old were fasted 6 h. Blood glucose was measured at -60 min with Glucometer prior dosing with compound or vehicle.

Functional Assay:

Article Title: Supporting Information Diamine Derivatives as Novel Small-Molecule, Potent, and Subtype- Selective Somatostatin SST3 Receptor Agonists
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.) SST cAMP Functional Assay Method: The cAMP agonist potency determinations were made using a modification of the PerkinElmer Lance 384 kit (Cat#AD0264). ..

Article Title: Investigation of Cardiovascular Effects of Tetrahydro-β-carboline sstr3 antagonists Supporting Information
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.) sstr cAMP Functional Assay Method: The cAMP agonist potency determinations were made using a modification of the PerkinElmer Lance 384 kit (Cat#AD0264). ..

Article Title: The Discovery of MK-4256, a Potent SSTR3 Antagonist as a Potential Treatment of Type-2 Diabetes Supporting Information
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.) SSTR cAMP Functional Assay Method: The cAMP agonist potency determinations were made using a modification of the PerkinElmer Lance 384 kit (Cat#AD0264). ..

Modification:

Article Title: Supporting Information Diamine Derivatives as Novel Small-Molecule, Potent, and Subtype- Selective Somatostatin SST3 Receptor Agonists
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.) SST cAMP Functional Assay Method: The cAMP agonist potency determinations were made using a modification of the PerkinElmer Lance 384 kit (Cat#AD0264). ..

Article Title: Investigation of Cardiovascular Effects of Tetrahydro-β-carboline sstr3 antagonists Supporting Information
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.) sstr cAMP Functional Assay Method: The cAMP agonist potency determinations were made using a modification of the PerkinElmer Lance 384 kit (Cat#AD0264). ..

Article Title: The Discovery of MK-4256, a Potent SSTR3 Antagonist as a Potential Treatment of Type-2 Diabetes Supporting Information
Article Snippet: .. Data were analyzed using Assay Data Analyzer software (Merck and Co.) SSTR cAMP Functional Assay Method: The cAMP agonist potency determinations were made using a modification of the PerkinElmer Lance 384 kit (Cat#AD0264). ..

Fluorescence:

Article Title: Supporting Information Diamine Derivatives as Novel Small-Molecule, Potent, and Subtype- Selective Somatostatin SST3 Receptor Agonists
Article Snippet: Plates were incubated for 45 minutes at 37°C and 5% CO2 followed by addition of 12 μL detection mix for 1 h incubation at room temperature Time-resolved fluorescence was measured using Envision plate reader (PerkinElmer Life and Analytical Sciences, Inc. Waltham, MA) at 665 nm and 615 nm. .. Resulting cAMP levels were calculated by reading the relative fluorescence units off a cAMP standard curve then agonist EC50s were subsequently determined from 4- parameter fits by plotting those cAMP levels versus agonist concentration using Assay Data Analyzer software (Merck and Co.). ..

Article Title: The Discovery of MK-4256, a Potent SSTR3 Antagonist as a Potential Treatment of Type-2 Diabetes Supporting Information
Article Snippet: Plates were incubated for 45 minutes at 37°C and 5% CO2 followed by addition of 12 μL detection mix for 1 h incubation at room temperature Time-resolved fluorescence was measured using Envision plate reader (PerkinElmer Life and Analytical Sciences, Inc. Waltham, MA) at 665 nm and 615 nm. .. Resulting cAMP levels were calculated by reading the relative fluorescence units off a cAMP standard curve then agonist EC50s were subsequently determined from 4-parameter fits by plotting those cAMP levels versus agonist concentration using Assay Data Analyzer software (Merck and Co.). .. Protocols for ipGTT and oGTT Mouse oGTT procedure C57BL/6 mice (Taconic), 12 weeks old were fasted 6 hrs.

Article Title: Investigation of Cardiovascular Effects of Tetrahydro-β-carboline sstr3 antagonists Supporting Information
Article Snippet: Plates were incubated for 45 minutes at 37°C and 5% CO2 followed by addition of 12 μL detection mix for 1 h incubation at room temperature Time-resolved fluorescence was measured using Envision plate reader (PerkinElmer Life and Analytical Sciences, Inc. Waltham, MA) at 665 nm and 615 nm. .. Resulting cAMP levels were calculated by reading the relative fluorescence units off a cAMP standard curve then agonist EC50s were subsequently determined from 4-parameter fits by plotting those cAMP levels versus agonist concentration using Assay Data Analyzer software (Merck and Co.). .. Protocol for mouse oGTT C57BL/6 mice (Taconic), 12 weeks old were fasted 6 h. Blood glucose was measured at -60 min with Glucometer prior dosing with compound or vehicle.

Concentration Assay:

Article Title: Supporting Information Diamine Derivatives as Novel Small-Molecule, Potent, and Subtype- Selective Somatostatin SST3 Receptor Agonists
Article Snippet: Plates were incubated for 45 minutes at 37°C and 5% CO2 followed by addition of 12 μL detection mix for 1 h incubation at room temperature Time-resolved fluorescence was measured using Envision plate reader (PerkinElmer Life and Analytical Sciences, Inc. Waltham, MA) at 665 nm and 615 nm. .. Resulting cAMP levels were calculated by reading the relative fluorescence units off a cAMP standard curve then agonist EC50s were subsequently determined from 4- parameter fits by plotting those cAMP levels versus agonist concentration using Assay Data Analyzer software (Merck and Co.). ..

Article Title: The Discovery of MK-4256, a Potent SSTR3 Antagonist as a Potential Treatment of Type-2 Diabetes Supporting Information
Article Snippet: Plates were incubated for 45 minutes at 37°C and 5% CO2 followed by addition of 12 μL detection mix for 1 h incubation at room temperature Time-resolved fluorescence was measured using Envision plate reader (PerkinElmer Life and Analytical Sciences, Inc. Waltham, MA) at 665 nm and 615 nm. .. Resulting cAMP levels were calculated by reading the relative fluorescence units off a cAMP standard curve then agonist EC50s were subsequently determined from 4-parameter fits by plotting those cAMP levels versus agonist concentration using Assay Data Analyzer software (Merck and Co.). .. Protocols for ipGTT and oGTT Mouse oGTT procedure C57BL/6 mice (Taconic), 12 weeks old were fasted 6 hrs.

Article Title: Investigation of Cardiovascular Effects of Tetrahydro-β-carboline sstr3 antagonists Supporting Information
Article Snippet: Plates were incubated for 45 minutes at 37°C and 5% CO2 followed by addition of 12 μL detection mix for 1 h incubation at room temperature Time-resolved fluorescence was measured using Envision plate reader (PerkinElmer Life and Analytical Sciences, Inc. Waltham, MA) at 665 nm and 615 nm. .. Resulting cAMP levels were calculated by reading the relative fluorescence units off a cAMP standard curve then agonist EC50s were subsequently determined from 4-parameter fits by plotting those cAMP levels versus agonist concentration using Assay Data Analyzer software (Merck and Co.). .. Protocol for mouse oGTT C57BL/6 mice (Taconic), 12 weeks old were fasted 6 h. Blood glucose was measured at -60 min with Glucometer prior dosing with compound or vehicle.



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(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
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(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
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(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
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https://www.bioz.com/product/assay+data+analyzer+software/analyzer+assay+data+software/pm24900466__ml200272z_si_001-52-4-8
Average 86 stars, based on 1 article reviews
assay data analyzer software - by Bioz Stars, 2026-10
86/100 stars
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(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad TC20). Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.

Journal: Cell reports

Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation

doi: 10.1016/j.celrep.2025.116758

Figure Lengend Snippet: (A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad TC20). Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.

Article Snippet: TC20 TM Data Analyzer Software , BioRad , https://www.bio-rad.com/webroot/web/html/lsr/products/cell_counting/product_overlay/global/tc20-data-ana.html.

Techniques: Flow Cytometry, Cell Characterization, Expressing, MANN-WHITNEY